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Cell viability, cell morphology, and nuclear morphology of butein against prostate cancer cell lines. ( A ) Chemical structure of butein. Formula: C 15 H 12 O 5 ; MW: 272.3. PC-3, DU145, and HPrEC cells were treated with butein at various concentrations (0, 1.25, 2.5, 5, 10, 15, 20, and 30 μM) and then cultured for 48 h. Viable cells were subjected to ( B ) trypan blue exclusion assay, and ( C ) MTT assay. ( D ) Cell morphology of PC-3 and DU145 cells was examined by phase contrast microscopy. ( E ) Nuclear morphology of PC-3 and DU145 cells was examined by fluorescence microscopy after DAPI (2 μg/mL) staining. All data are expressed as mean ± standard error of the mean of three independent experiments. *, p < 0.05 was compared to respective controls. HPrEC: human prostate epithelial cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; and Conc., concentration.

Journal: Life

Article Title: Effect of Butein, a Plant Polyphenol, on Apoptosis and Necroptosis of Prostate Cancer Cells in 2D and 3D Cultures

doi: 10.3390/life15060836

Figure Lengend Snippet: Cell viability, cell morphology, and nuclear morphology of butein against prostate cancer cell lines. ( A ) Chemical structure of butein. Formula: C 15 H 12 O 5 ; MW: 272.3. PC-3, DU145, and HPrEC cells were treated with butein at various concentrations (0, 1.25, 2.5, 5, 10, 15, 20, and 30 μM) and then cultured for 48 h. Viable cells were subjected to ( B ) trypan blue exclusion assay, and ( C ) MTT assay. ( D ) Cell morphology of PC-3 and DU145 cells was examined by phase contrast microscopy. ( E ) Nuclear morphology of PC-3 and DU145 cells was examined by fluorescence microscopy after DAPI (2 μg/mL) staining. All data are expressed as mean ± standard error of the mean of three independent experiments. *, p < 0.05 was compared to respective controls. HPrEC: human prostate epithelial cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; and Conc., concentration.

Article Snippet: HPrEC cells were cultured in Prostate Epithelial Cell Base Medium (ATCC, PCS-440-030) supplemented with (ATCC, PCS-440-040).

Techniques: Cell Culture, Trypan Blue Exclusion Assay, MTT Assay, Microscopy, Fluorescence, Staining, Concentration Assay